Fig 2: PBNP-PTT triggers greater immunophenotypic changes in MYCN-non-amplified SH-SY5Y cells than MYCN-amplified LAN-1 neuroblastoma cell line in vitro. SH-SY5Y (blue) and LAN-1 (green) cells were treated with varied thermal doses via PBNP-PTT and analyzed for (A) % live cells (B) intracellular ATP, (C) secreted HMGB1, and cell surface expression levels of (D) calreticulin, (E) CD80, (F) CD86, (G) PD-L1, (H) B7-H3, (I) HLA-ABC, (J) HLA-DR, (K) PVR, and (L) GD2. Data represent mean ± SD (n = 2 independent samples).

Fig 3: Co-incubation of treated HPV-positive and HPV-negative HNSCC tumor cells had no significant effect on the expression of activation markers of hmDCs. 24 h after the last treatment, the cells were harvested and co-cultured with hmDCs. 48 h after the co-incubation the hmDCs were analyzed using flow cytometry. A: Presenting the gating strategy. B-E: For the hmDCs, which were co-cultured with the HPV-negative cell lines Cal33 and HSC-4, as well as HPV-positive cell lines UD-Scc-2 and UM-Scc-47, the mean fluorescence intensity (ΔMFI) of CD70 (B), CD80 (C), CD83 (D) and CD86 (E) is shown as stacked bars showing the mean with ±SD. A Mann-Whitney test was calculated to compare hmDCs without maturation cocktail and hmDCs with maturation cocktail. Furthermore, a Kruskal-Wallis test with Dunn's correction for multiple testing was calculated to compare the different treatment approaches versus hmDCs without maturation cocktail. *p < 0.05, **p < 0.01, ***p < 0.001; n≥5.